Sialoadhesin/CD169 抗体
Quick Overview for Sialoadhesin/CD169 抗体 (ABIN317446)
抗原
See all Sialoadhesin/CD169 (SIGLEC1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognises Mouse CD169, a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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产品特性
- Synonyms: Sialoadhesin, Siglec-1, Sialic acid-binding Ig-like lectin 1
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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免疫原
- Purified Sialoadhesin. Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cellline.
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亚型
- IgG2a
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anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody
SIGLEC1 适用: 人, 大鼠 FACS, ELISA, IHC (fro), ICC, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 20-197) antibodySIGLEC1 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2A4D1 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (APC)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 APC
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 大鼠 FACS, IP, IHC (fro), IF 宿主: 小鼠 Monoclonal ED3 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibodySIGLEC1 适用: 人 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 7-239 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) antibody (PE)SIGLEC1 适用: 人 FACS 宿主: 小鼠 Monoclonal 7-239 PE
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 851-950) antibody (AbBy Fluor® 647)SIGLEC1 适用: 人, 大鼠 FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 647
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (Internal Region) antibodySIGLEC1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (AA 50-300) antibodySIGLEC1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1) (N-Term) antibodySIGLEC1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal HSn 7D2 unconjugated
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应用备注
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Immunohistochemistry on Frozen Sections: 1/50-1/100 (Fixation with either 2 % paraformaldehyde or ethanol is recommended). Flow Cytometry: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Sialoadhesin/CD169 (SIGLEC1) (Sialic Acid Binding Ig-Like Lectin 1, Sialoadhesin (SIGLEC1))
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别名
- CD169 / SIGLEC1
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背景
- Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker, myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells, CD33, a myeloid differentiation antigen, and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.Synonyms: Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec-1
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基因ID
- 20612
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UniProt
- Q62230
抗原
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